(S)-N-acetyldemethylphosphinothricin (NAcDMPT) is a catalyst and methyl acceptor for cobalamin-dependent methyltransferases (Class B Radical SAM methyltransferases (RSMTs) (Werner et al., 2011; Zhang, Van Der Donk, & Liu, 2012). In Streptomyces hygroscopicus, Streptomyces viridochromogenes, and Kitasatospora phosalacinea, this reaction produces L-Phosphinothricin, or glufosinate, an amino acid and glutamate analog (Allen & Wang, 2014; Hu, Werner, Allen, & Wang, 2015; Li et al., 2015). Glufosinate and its derivatives are only molecules in nature known to contain C-P-C linkages (Werner et al., 2011). L-phosphinothricin has herbicidal and antimicrobial properties through its ability to inhibit glutamate synthesis (Werner et al., 2011) and is the key to Liberty Link herbicide resistance in crops.Class B RSMTs are heavily linked to antibiotic biosynthesis (Werner et al., 2011). As a precursor to L-phosphinothricin, NAcDMPT is used to study PhpK and other RSMTs. Recent research suggests Shewanella denitrificans also catalyzes a L-Phosphinothricin reaction to produce a phosphinate natural product (Allen & Wang, 2014). NAcDMPT and other methyl acceptors like DMPT (ASIS-0069) are useful tools for evaluating the activity of RSMTs and may enable the discovery of novel antimicrobials.